Journal: Science Progress

Article Title: Low-dose urapidil mitigates renal ischemia-reperfusion injury through matrix metalloproteinase-9 inhibition and anti-inflammatory effects

doi: 10.1177/00368504261438886

Figure Lengend Snippet: Comparison of MMP-9 IHC between the kidney samples from each group. Details of MMP-9 marking were observed with IHC. MMP-9, matrix metalloproteinase-9; IHC, immunohistochemistry; SH, sham group; CR, control group; I/R, ischemia-reperfusion injury; UP, urapidil.

Article Snippet: Following the completion of the blocking procedure, the samples were treated with primary antibodies, including eNOS (Boster Bio., catalog number: A01604-2, diluted at a ratio of 1:250), caspase 3 (Thermo Scıentıfıc, catalog number: RB-1197-P0, diluted at a ratio of 1:100), TNF-α (Proteintech, catalog number:60291-1-Ig, diluted at a ratio of 1:300), IL-1β (Bioss, catalog number:bs-6319R, diluted at a ratio of 1:150), IL-6 (ST John’s, catalog number:STJ1110424, diluted at a ratio of 1:100), and MMP-9 (Boster Bio, catalog number: PA2140-2, diluted at a ratio of 1:100).

Techniques: Comparison, Immunohistochemistry, Control

Journal: The Journal of pathology

Article Title: A 3D tri-culture system reveals that activin receptor-like kinase 5 and connective tissue growth factor drive human glomerulosclerosis

doi: 10.1002/path.4960

Figure Lengend Snippet: (A) MC 3D monoculture without the addition of TGFβ, and (B) in the presence of TGFβ, shown by H&E staining of paraffin-embedded sections. Human collagen type I/III (immunostained with an antibody that recognises both collagen type I and III) (C) and human collagen type IV (D) can be demonstrated in nodules by immunoperoxidase staining. (E) High-power view of MC nodule by scanning electron microscopy in cross section. (F) MC nodule count and collagen type I alpha1 (COL1a1), and collagen type IV alpha1 (COL4a1) RNA quantification (n=3). (G) Effect of ALK5 inhibition (Alk5i) on MC nodule formation (n=3). (H) Effect of SMAD2 or SMAD3 siRNA knockdown in MCs on nodule formation (n=3) with immunoblots demonstrating degree of knockdown. [DharmaFECT (DH), non-targeting siRNA control (siCP), siSMAD2 (siS2), siSMAD3 (siS3), total SMAD2 (tSMAD2), total SMAD3 (tSMAD3)]. Error bars represent 1 s.d. NS, not significant; ***, p<0.001; **, p<0.01: 2-tailed Student’s t test.

Article Snippet: After culture, matrices were embedded in HistoGel, fixed in 4% paraformaldehyde and paraffin wax-embedded for sectioning and staining for human collagen type IV (mouse anti-human collagen IV monoclonal antibody 2150–0121, Bio-Rad, CA, USA) and human collagen type I/III (rabbit anti-human collagen I/III polyclonal antibody 2150–2210, Bio-Rad, CA, USA).

Techniques: Staining, Immunoperoxidase Staining, Electron Microscopy, Inhibition, Western Blot